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Attempt the following questions to check your understanding of the subject:
PART 1
- Explain the following terms used in cloning
- DNA recombination (3 marks)
- Transformation (3 marks)
- selective amplification (3 marks)
- cloning vectors (3 marks)
- List and explain the reasons for the wide application of micro-organisms in food biotechnology processes (6 marks)
- Explain the ethical considerations in modern biotechnology application (6 marks)
- Define the term mutagenesis and explain different types of mutations. (6 marks)
- Discuss safety and health issues associated with modern biotechnology (6 marks)
- State the advantages of tissue culture over intact plants (6 marks)
PART 2
- State and explain the factors affecting fermentation (10 marks)
- State and explain any five enzymes used in recombinant DNA technology (10 marks)
- Explain application of re-compliant DNA technology in environmental protection (6 marks)
- Compare solid substance culture and submerged liquid culture. (10 marks)
- Describe the polymerase chain reaction procedure and its applications (10 marks)